The Swi3 Protein Plays A Unique Role In Regulating Respiration In Eukaryotes

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The Swi3 Protein Plays A Unique Role In Regulating Respiration In Eukaryotes

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Title: The Swi3 Protein Plays A Unique Role In Regulating Respiration In Eukaryotes
Author(s):
Lal, Sneha;
Alam, Md Maksudul;
Hooda, Jagmohan;
Shah, Ajit;
Cao, Thai M.;
Xuan, Zhenyu;
Zhang, Li
Item Type: article
Keywords: Show Keywords
Abstract: Recent experimental evidence increasingly shows that the dysregulation of cellular bioenergetics is associated with a wide array of common human diseases, including cancer, neurological diseases and diabetes. Respiration provides a vital source of cellular energy for most eukaryotic cells, particularly high energy demanding cells. However, the understanding of how respiration is globally regulated is very limited. Interestingly, recent evidence suggests that Swi3 is an important regulator of respiration genes in yeast. In this report, we performed an array of biochemical and genetic experiments and computational analysis to directly evaluate the function of Swi3 and its human homologues in regulating respiration. First, we showed, by computational analysis and measurements of oxygen consumption and promoter activities, that Swi3, not Swi2, regulates genes encoding functions involved in respiration and oxygen consumption. Biochemical analysis showed that the levels of mitochondrial respiratory chain complexes were substantially increased in Delta swi3 cells, compared with the parent cells. Additionally, our data showed that Swi3 strongly affects haem/oxygen-dependent activation of respiration gene promoters whereas Swi2 affects only the basal, haem-independent activities of these promoters. We found that increased expression of aerobic expression genes is correlated with increased oxygen consumption and growth rates in Delta swi3 cells in air. Furthermore, using computational analysis and RNAi knockdown, we showed that the mammalian Swi3 BAF155 and BAF170 regulate respiration in HeLa cells. Together, these experimental and computational data demonstrated that Swi3 and its mammalian homologues are key regulators in regulating respiration.
ISSN: 0144-8463
Source: Bioscience Reports
Link to Related Resource: http://dx.doi.org/10.1042/BSR20160083
Persistent Link: http://hdl.handle.net/10735.1/5980
Bibliographic Citation: Lal, Sneha, Md Maksudul Alam, Jagmohan Hooda, Ajit Shah, et al. 2016. "The Swi3 protein plays a unique role in regulating respiration in eukaryotes." Bioscience Reports 36(3), doi:10.1042/BSR20160083
Terms of Use: CC BY 4.0 (Attribution)
©2016 The Authors
Sponsors: Cecil H. and Ida Green funds grant number R15GM107717

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CC BY 4.0 (Attribution) Except where otherwise noted, this item's license is described as CC BY 4.0 (Attribution)