A Novel Approach for Electrical Tuning of Nano-Textured Zinc Oxide Surfaces for Ultra-Sensitive Troponin-t Detection

DSpace/Manakin Repository

A Novel Approach for Electrical Tuning of Nano-Textured Zinc Oxide Surfaces for Ultra-Sensitive Troponin-t Detection

Show full item record

Title: A Novel Approach for Electrical Tuning of Nano-Textured Zinc Oxide Surfaces for Ultra-Sensitive Troponin-t Detection
Author(s):
Munje, Ruijuta D. (UT Dallas);
Jacobs, Michael (UT Dallas);
Muthukumar, S.;
Quadri, Bilal (UT Dallas);
Shanmugam, Nandhinee Radha (UT Dallas);
Prasad, Shalini (UT Dallas)
Item Type: article
Keywords: Show Keywords
Abstract: We have developed a label-free, non-faradaic, electrochemical sensor for ultra-sensitive detection of a cardiac biomarker, troponin-T by utilizing the stoichiometric surface compositions of nanotextured zinc oxide (ZnO) thin films. In this study, we show how the performance of a nanotextured zinc oxide based non-faradaic biosensor is modulated by differences in the fabrication parameters of the metal oxide thin film as well as the choice of cross-linkers. Two cross-linking molecules, dithiobis succinimidyl propionate and 3-aminopropyl triethoxysilane, demonstrate significantly different binding chemistries with zinc oxide. The non-faradaic electrochemical behaviour of the sensor due to the two linkers is compared by analyzing the troponin-T dose response using electrochemical impedance spectroscopy (EIS). The sensor performance associated with both linkers is compared based on the dynamic range and limit of detection. The sensor utilizing zinc surface terminations demonstrated a wider dynamic range between the two linkers. This range extended from 26% to 54% in phosphate buffered saline and from 21% to 65% in human serum, for a concentration range from 10 fg mL⁻¹ to 1 ng mL⁻¹ of troponin-T. The limit of detection was found to be at 10 fg mL⁻¹ and has potential utility in the development of point-of-care (POC) diagnostics for cardiovascular diseases. Fluorescence quantification analysis was also performed to further validate the specificity of the linker binding to the ZnO films. An ultrasensitive troponin-T biosensor can be designed by leveraging the zinc termination based surface chemistry for selective protein immobilization.
Publisher: The Royal Society of Chemistry
ISSN: 1759-9660 (ISSN)
Persistent Link: http://dx.doi.org/10.1039/c5ay02052b
http://hdl.handle.net/10735.1/4809
Bibliographic Citation: Munje, R. D., M. Jacobs, S. Muthukumar, B. Quadri, N. R. Shanmugam, et al. 2015. "A novel approach for electrical tuning of nano-textured zinc oxide surfaces for ultra-sensitive troponin-T detection." Analytical Methods 7(24): 10136-10144.
Terms of Use:
©2015 The Royal Society of Chemistry. This article may not be further made available or distributed.

Files in this item

Files Size Format View
JECS-3738-273736.19.pdf 977.0Kb PDF View/Open Article

This item appears in the following Collection(s)


Show full item record