A Novel Virus-Inducible Enhancer of the Interferon-Beta Gene with Tightly Linked Promoter and Enhancer Activities

DSpace/Manakin Repository

A Novel Virus-Inducible Enhancer of the Interferon-Beta Gene with Tightly Linked Promoter and Enhancer Activities

Show full item record

Title: A Novel Virus-Inducible Enhancer of the Interferon-Beta Gene with Tightly Linked Promoter and Enhancer Activities
Author(s):
Banerjee, A. Raja;
Kim, Yoon Jung (UT Dallas);
Kim, Tae Hoon (UT Dallas)
Item Type: Article
Keywords: Show Keywords
Description: Supplementary data available at DOI.
Abstract: Long-range enhancers of transcription are a key component of the genomic regulatory architecture. Recent studies have identified bi-directionally transcribed RNAs emanating from these enhancers known as eRNAs. However, it remains unclear how tightly coupled eRNA production is with enhancer activity. Through our systematic search for long-range elements that interact with the interferon-beta gene, a model system for studying inducible transcription, we have identified a novel enhancer, which we have named L2 that regulates the expression of interferon-beta. We have demonstrated its virus-inducible enhancer activity by analyzing epigenomic profiles, transcription factor association, nascent RNA production and activity in reporter assays. This enhancer exhibits intimately linked virus-inducible enhancer and bidirectional promoter activity that is largely dependent on a conserved Interferon Stimulated Response Element and robustly generates virus inducible eRNAs. Notably, its enhancer and promoter activities are fully retained in reporter assays even upon a complete elimination of its associated eRNA sequences. Finally, we show that L2 regulates IFNB1 expression by siRNA knockdown of eRNAs, and the deletion of L2 in a BAC transfection assay. Thus, L2 is a novel enhancer that regulates IFNB1 and whose eRNAs exert significant activity in vivo that is distinct from those activities recapitulated in the luciferase reporter assays.
ISSN: 0305-1048
Persistent Link: http://hdl.handle.net/10735.1/4379
http://dx.doi.org/10.1093/nar/gku1018
Terms of Use: CC-BY 4.0 (Attribution)
©2014 The Authors
Sponsors: National Institute of Allergy and Infectious Diseases [R21AI107067]; National Cancer Institute [R01CA140485]; National Institutes of Health Training Grant [T32M007499]. Funding for open access charge: National Institute of Allergy and Infectious Diseases [R21AI107067].

Files in this item

Files Size Format View
NSM-4378-271949.49.pdf 1.767Mb PDF View/Open Article

This item appears in the following Collection(s)


Show full item record

CC-BY 4.0 (Attribution) Except where otherwise noted, this item's license is described as CC-BY 4.0 (Attribution)