Characterizing the strand-specific distribution of non-CpG methylation in human pluripotent cells

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Characterizing the strand-specific distribution of non-CpG methylation in human pluripotent cells

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Title: Characterizing the strand-specific distribution of non-CpG methylation in human pluripotent cells
Author(s):
Guo, Weilong;
Chung, Wen-Yu;
Qian, Minping;
Pellegrini, Matteo;
Zhang, Michael Q.
Date Created: 2013-12-16
Format: Text
Item Type: Article
Keywords: DNA--Methylation
Introns
CpG (cytidine-phosphate-guanosine)
Abstract: DNA methylation is an important defense and regulatory mechanism. In mammals, most DNA methylation occurs at CpG sites, and asymmetric non-CpG methylation has only been detected at appreciable levels in a few cell types. We are the first to systematically study the strand-specific distribution of non-CpG methylation. With the divide-and-compare strategy, we show that CHG and CHH methylation are not intrinsically different in human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We also find that non-CpG methylation is skewed between the two strands in introns, especially at intron boundaries and in highly expressed genes. Controlling for the proximal sequences of non-CpG sites, we show that the skew of non-CpG methylation in introns is mainly guided by sequence skew. By studying subgroups of transposable elements, we also found that non-CpG methylation is distributed in a strand-specific manner in both short interspersed nuclear elements (SINE) and long interspersed nuclear elements (LINE), but not in long terminal repeats (LTR). Finally, we show that on the antisense strand of Alus, a non-CpG site just downstream of the A-box is highly methylated. Together, the divide-and-compare strategy leads us to identify regions with strand-specific distributions of non-CpG methylation in humans.;
Publisher: Oxford University Press
ISSN: 1362-4962
Source: Nucleic Acids Research
Link to Related Resource: http://dx.doi.org/10.1093/nar/gkt1306
Persistent Link: http://hdl.handle.net/10735.1/3700
Bibliographic Citation: Guo, Weilong, Wen-Yu Chung, Minping Qian, Matteo Pellegrini, et al. 2014. "Characterizing the strand-specific distribution of non-CpG methylation in human pluripotent cells." Nucleic Acids Research 42(5): 3009-3016.
Terms of Use: CC BY-NC 3.0 (Attribution-NonCommercial)
©2013 The Authors.

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