Integrated Omics Study Delineates the Dynamics of Lipid Droplets in Rhodococcus Opacus PD630

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Integrated Omics Study Delineates the Dynamics of Lipid Droplets in Rhodococcus Opacus PD630

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Title: Integrated Omics Study Delineates the Dynamics of Lipid Droplets in Rhodococcus Opacus PD630
Author(s):
Chen, Yong;
Ding, Yunfeng;
Yang, Li;
Yu, Jinhai;
Liu, Guiming;
Wang, Xumin;
Zhang, Shuyan;
Zhang, Michael Q. (UT Dallas);
Li, Yanda
Format: Text
Item Type: Article
Keywords: Show Keywords
Description: Includes supplementary material.
Abstract: Rhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.
Publisher: Oxford University Press
ISSN: 0305-1048
Persistent Link: http://hdl.handle.net/10735.1/3684
https://dx.doi.org/10.1093/nar/gkt932
Terms of Use: ©2013 The Authors. "This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited."

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