A Carbon Nanotube-based Raman-imaging Immunoassay For Evaluating Tumor Targeting Ligands

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A Carbon Nanotube-based Raman-imaging Immunoassay For Evaluating Tumor Targeting Ligands

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Title: A Carbon Nanotube-based Raman-imaging Immunoassay For Evaluating Tumor Targeting Ligands
Author(s):
Bajaj, Pooja;
Mikoryak, Carole;
Wang, Ruhung;
Bushdiecker ⅠⅠ, David K.;
Memon, Pauras;
Draper, Rockford K.;
Dieckmann, Gregg R.;
Pantano, Paul (UT Dallas);
Musselman, Inga H.
Format: Text
Item Type: Article
Keywords: Carbon nanotubes
Cell receptors
Breast--Cancer--Research
Abstract: Herein, we describe a versatile immunoassay that uses biotinylated single-walled carbon nanotubes (SWNTs) as a Raman label, avidin-biotin chemistry to link targeting ligands to the label, and confocal Raman microscopy to image whole cells. Using a breast tumor cell model, we demonstrate the usefulness of the method to assess membrane receptor/ligand systems by evaluating a monoclonal antibody, Her-66, known to target the Her2 receptors that are overexpressed on these cells. We present two-dimensional Raman images of the cellular distribution of the SWNT labels corresponding to the distribution of the Her2 receptors in different focal planes through the cell with validation of the method using immunofluorescence microscopy, demonstrating that the Her-66-SWNT complexes were targeted to Her2 cell receptors.;
Publisher: Royal Society of Chemistry
ISSN: 1364-5528
Persistent Link: http://hdl.handle.net/10735.1/3670
http://dx.doi.org/10.1039/c4an00258j
Terms of Use: ©2014 The Royal Society of Chemistry. This article may not be further copied or distributed.

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