An Assay of DNA by Resonance Light Scattering Technique and its Application in Screening Anticancer Drugs

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An Assay of DNA by Resonance Light Scattering Technique and its Application in Screening Anticancer Drugs

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Title: An Assay of DNA by Resonance Light Scattering Technique and its Application in Screening Anticancer Drugs
Author(s):
Chen, Xi.;
Liu, G.;
Liang, S.;
Qian, S.;
Liu, Jinbin;
Chen, Z.
Date Created: 2011-12-22
Format: text
Item Type: article
Keywords: DNA
Bromine compounds
Drug interactions
Light--Scattering
Abstract: In this contribution, a novel assay has been developed to detect the interaction between DNA and drugs using ethidium bromide (EB) as a probe based on the decreased resonance light scattering (RLS) technique. It was found that, in pH 2.0 Britton-Robinson buffer solution, EB underwent dramatic enhancement of RLS signal when binding to DNA. Additionally, the strong RLS signal of the EB-DNA system was remarkably decreased after the addition of DNA-targeted anticancer drugs. The results showed a linear relationship between the enhanced RLS intensity of the EB-DNA system and concentration of DNA in the range of 0.015-40.8 μg mL -1. The linear regression equation is represented as follows: ΔI RLS = 4.15 + 21.14c with regression coefficient r = 0.9970 (n = 10). The detection limit was 0.3 ng mL -1. Only 2.0 × 10 -8 mol L -1 was selected as the optimum EB concentration in this assay. Further study demonstrated that the anticancer efficacy of anticancer drugs varied inversely with RLS intensity of the EB-DNA-drug system. So, it is intuitive to see the sequence of antitumor efficiency of anticancer drugs without data processing by RLS screening spectra. Compared with the traditional cell-based screening methods, the proposed method was more convenient, rapid and intuitive. The reaction mechanism is discussed. © 2012 The Royal Society of Chemistry.
ISSN: 1759-9660
Source: Analytical Methods
Link to Related Resource: http://dx.doi.org/10.1039/c2ay05556b
Persistent Link: http://hdl.handle.net/10735.1/3163
Bibliographic Citation: Chen, Xi, Guoliang Liu, Shengwang Liang, Sihua Qian, et al. 2012. "An assay of DNA by resonance light scattering technique and its application in screening anticancer drugs." Analytical Methods 4(6): 1546-1551.
Terms of Use: © 2012 The Royal Society of Chemistry

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