The Nuclear Localization of SWI/SNF Proteins Is Subjected to Oxygen Regulation

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The Nuclear Localization of SWI/SNF Proteins Is Subjected to Oxygen Regulation

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Title: The Nuclear Localization of SWI/SNF Proteins Is Subjected to Oxygen Regulation
Author(s):
Dastidar, Ranita Ghosh;
Hooda, Jagmohan;
Shah, Ajit;
Cao, Thai M.;
Robert Michael Henke;
Zhang, Li (UT Dallas)
Date Created: 2012-07-13
Format: Text
Item Type: Article
Keywords: Show Keywords
Description: Funding provided by grants from NIH (GM62246) and NCI Cancer Center (1P30 CA142543-01).
Abstract: Background: Hypoxia is associated with many disease conditions in humans, such as cancer, stroke and traumatic injuries. Hypoxia elicits broad molecular and cellular changes in diverse eukaryotes. Our recent studies suggest that one likely mechanism mediating such broad changes is through changes in the cellular localization of important regulatory proteins. Particularly, we have found that over 120 nuclear proteins with important functions ranging from transcriptional regulation to RNA processing exhibit altered cellular locations under hypoxia. In this report, we describe further experiments to identify and evaluate the role of nuclear protein relocalization in mediating hypoxia responses in yeast.Results: To identify regulatory proteins that play a causal role in mediating hypoxia responses, we characterized the time courses of relocalization of hypoxia-altered nuclear proteins in response to hypoxia and reoxygenation. We found that 17 nuclear proteins relocalized in a significantly shorter time period in response to both hypoxia and reoxygenation. Particularly, several components of the SWI/SNF complex were fast responders, and analysis of gene expression data show that many targets of the SWI/SNF proteins are oxygen regulated. Furthermore, confocal fluorescent live cell imaging showed that over 95% of hypoxia-altered SWI/SNF proteins accumulated in the cytosol in hypoxic cells, while over 95% of the proteins were nuclear in normoxic cells, as expected.Conclusions: SWI/SNF proteins relocalize in response to hypoxia and reoxygenation in a quick manner, and their relocalization likely accounts for, in part or in whole, oxygen regulation of many SWI/SNF target genes.
ISSN: 2045-3701
Persistent Link: http://hdl.handle.net/10735.1/3126
http://dx.doi.org/10.1186/2045-3701-2-30
Terms of Use: © 2012 Ghosh Dastidar et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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