Engineering Multiple Site-Specific Modifications in Supercoiled DNAs

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Engineering Multiple Site-Specific Modifications in Supercoiled DNAs

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Title: Engineering Multiple Site-Specific Modifications in Supercoiled DNAs
Author(s):
Bharadwaj, Anusha (UT Dallas);
Kesinger, Matthew R. (UT Dallas);
Shoura, Massa J. (UT Dallas);
Vetcher, Alexandre (UT Dallas);
Levene, Stephen D. (UT Dallas)
Sponsors: This work was supported by grants from the DMS/NIGMS Joint Program in Mathematical Biology (GM67242 and DMS-800929).
Format: Text
Item Type: Presentation
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Abstract: New techniques are needed in order to study the effects of DNA supercoiling on loop-mediated regulation. The thermodynamics of DNA looping depend periodically on the helical phasing between recognition sites. A long-term goal is to provide enabling technologies for ensemble and single-molecule FRET studies of DNA looping mediated by lac repressor in vitro and in vivo. Other applications of the methodology include engineering of DNA substrates for studies of site-specific recombination and other enzyme systems.
Persistent Link: http://hdl.handle.net/10735.1/2141

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