DNA Looping in Topologically Constrained Domains

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DNA Looping in Topologically Constrained Domains

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Title: DNA Looping in Topologically Constrained Domains
Author(s):
Bharadwaj, Anusha (UT Dallas);
Kesinger, Matthew R. (UT Dallas);
Shoura, Massa J. (UT Dallas);
Vetcher, Alexandre (UT Dallas);
Levene, Stephen D. (UT Dallas)
Sponsors: This work was supported by grants from the DMS/NIGMS Joint Program in Mathematical Biology (GM67242 and DMS-800929).
Format: Text
Item Type: Article
Presentation
Keywords: DNA
Fluorescence resonance energy transfer
Recombination, genetic
DNA-protein interactions
Abstract: New techniques are needed in order to study the effects of DNA supercoiling on loop-mediated regulation. The thermodynamics of DNA looping depend periodically on the helical phasing between recognition sites. A long-term goal is to provide enabling technologies for ensemble and single-molecule FRET studies of DNA looping mediated by lac repressor in vitro and in vivo. Other applications of the methodology include engineering of DNA substrates for studies of site-specific recombination and other enzyme systems
Publisher: Oxford University Press.
Persistent Link: http://hdl.handle.net/10735.1/4043
http://dx.doi.org/10.1093/nar/gks430
Bibliographic Citation: Shoura, M. J., A. A. Vetcher, S. M. Giovan, F. Bardai, A. Bharadwaj, M. R. Kesinger, and S. D. Levene. 2012. "Measurements of DNA-Loop Formation Via Cre-Mediated Recombination." Nucleic Acids Research 40 (15): 7452-7464.
Terms of Use: CC BY-NC 4.0 (Attribution-NonCommercial) License
©2012 The Authors

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